A Brief Introduction of High-throughput Sequencing High-throughput sequencing, also known as next-generation sequencing (NGS), is a technique that sequences hundreds of thousands to millions of nucleic acid molecules at a time, as opposed to traditional first-generation sequencing (Sanger sequencing). This technology prepares a sequencing library by randomly fragmenting and splicing DNA, and finally obtains sequence information by performing extension reactions on tens of thousands of clones in the library and detecting the corresponding signals.