Recently, the news of "$1.2 million anti-cancer drug" has pushed CAR-T therapy to the people. Most people are more curious and have questions about this new therapy than about its expensive price.The principle of CAR-T therapy is not difficult to understand. Simply, it is the modification of T-lymph
Nucleic acid purification has been widely used in molecular biology laboratories. How to obtain high quality and high purity DNA or RNA samples is crucial to the smooth performance of downstream experiments. Therefore, the identification of purified nucleic acids is also an essential step.
Analysis of Four Conventional Nucleic Acid Purification Methods From the development history of nucleic acid extraction technology, nucleic acid extraction has gone through four main development stages.② Precipitation method with organic solvent extraction technology. ③ Spin column method with
Proteinase K is a powerful proteolytic enzyme isolated from Candida albicans that can digest natural Keratin, hence the name Proteinase K. It belongs to the S8 family of Serine Protease SB Superfamily. The main cleavage site of Proteinase K is the carboxy-terminal peptide bond of aliphatic and aromatic amino acids, which has high specific activity and is a key reagent for DNA extraction.