This product uses the dye qPCR method (SYBR Green I) for accurate quantification of libraries in the Illumina sequencing process. The kit provides DNA reference standards, SYBR qPCR Master Mix, amplification primers and the reference dye ROX. The reference standard contains six double-stranded DNA fragment solutions at a concentration of 0.0002pM-20pM; the amplification primers are designed based on NGS library junction sequences P5 and P7 to ensure specific amplification of library molecules with intact double-ended junctions; SYBR qPCR Master Mix is a fuel-based qPCR premix with antibody-based hot start, which exhibits efficient and specific amplification in samples of different lengths and GC contents, and is well suited for absolute quantification of library concentration.
High amplification efficiency, good GC content compatibility
Compatible with all mainstream quantitative PCR instruments
Strict quality control to ensure maximum batch-to-batch stability