Plasma microbiome cell-free DNA (mcfDNA) is a potential biomarker for the diagnosis of the etiology of infection. In the absence of samples from infection foci, the detection of plasma mcfDNA brings important value for pathogen identification.
BunnyMag Plasma Microbiome cfDNA Isolation Kit adopts advanced nucleic acid extraction technology with superparamagnetic beads and a unique buffer system to rapidly extract mcfDNA from fresh or frozen plasma, easily achieving low concentration of trace nucleic acid extraction. The extraction process of this kit is safe and non-toxic, with maximum removal of proteins, lipids and other impurities. The isolated DNA is suitable for a variety of downstream applications, including PCR, ddPCR, qPCR, mNGS library preparation, etc., as verified by hundreds of clinical samples.
High Quality: The extraction process does not require Carrier RNA, effectively removes impurities and downstream PCR inhibition factors and facilitates accurate quantification.
Unbiased: Unbiased microbial nucleic acid extraction, compatible with multiple pathogen types, minimize microbial loss in samples.
Low Microbial Contamination: Effectively control contamination by exogenous pathogenic micro-organisms.
Broad Compatibility: Compatible with manual and automatic extraction programs, adapted to Tecan, Hamilton, PE and other nucleic acid extraction workstations.
Figure 1-2. Plasma mcfDNA extraction and library preparation with high consistency with competitive products
mcfDNA was extracted from plasma samples using BunnyMag Plasma Microbiome cfDNA Isolation Kit and Qiagen's comparable products, and library preparation was performed at equal quality. The extraction yield and library preparation yield of BunnyMag kits were consistent with those of competing products.
Lysis Binding Buffer
Guanidine isothiocyanate, 5 M
Washing Buffer 1
Guanidine isothiocyanate, 3 M
Washing Buffer 2
Ethanol absolute, 80%
20 mg/mL Proteinase K
Please take out the magnetic beads and proteinase K and store at 2-8℃, and others store at 10-30℃.
The validity period is 12 months.