FFPE is a formalin-fixed, paraffin-embedded sample that can be retained at room temperature for a long time. The study of these tissue samples provides a favorable basis for exploring new criteria for tumor classification, diagnosis and prognosis, developing experimental techniques for early tumor detection, translational medicine and individualized medicine.Formalin-fixed paraffin embedding (FFPE) is a common preservation method for clinical tissue samples. BunnyMag FFPE DNA Isolation Kit uses nano-magnetic beads with unique separation and a specially designed buffer system to extract high-quality genomic DNA from FFPE samples.
25 tests/ 50 tests/ 100 tests/ 200 tests
BunnyMag FFPE DNA Isolation Kit provides a simple, rapid and efficient method to extract genomic DNA from paraffin-embedded tissues, paraffin-sectioned tissues and paraffin-fixed samples. Paraffin tissues are dewaxed and treated with efficient lysis reagents to release DNA from the tissues, and then bound to the surface of magnetic beads by fast magnetic response, and finally highly purified genomic DNA is obtained, which is validated by hundreds of real clinical samples and can be directly used for a variety of downstream experiments such as PCR, qPCR and library preparation.
High Purity: Extraction of genomic DNA with high yield, high purity and low protein and RNA contamination.
Various Sample Type: Suitable for the extraction of various FFPE tissue samples and low-quality samples.
Safe: Simple operation, no phenol, chloroform and other organic solvents.
Broad Compatibility: Compatible with manual and automated extraction process.
Figure 1. High yield of DNA extraction from FFPE samples.
Using BunnyMag FFPE DNA Isolation Kit and similar products from Qiagen and ThermoFisher, DNA extraction was performed on 10 clinical FFPE samples respectively, and the DNA extraction yield of this product was better than similar products from competing companies.
Figure 2. High DNA quality and consistent fragment distribution with competing products.
Using BunnyMag FFPE DNA Isolation Kit and similar products from Qiagen and ThermoFisher, DNA is extracted from FFPE samples for library preparation. The library was analyzed by Agilent 2100 Bioanalyzer and the fragment size was highly consistent with competing products.
Lysis Binding Buffer
Washing Buffer 1
Ethanol absolute, 50%-80%
Washing Buffer 2
Ethanol absolute, 50%-80%
20 mg/mL Proteinase K
Please take out the magnetic beads and proteinase K and store at 2-8℃, and others store at 10-30℃.
The validity period is 12 months.