SPRI (Solid Phase Reverse Immobilization), uses paramagnetic beads to selectively bind nucleic acids by type and size, which can be used to isolate, purify and clean up high purity nucleic acids. The technology can be applied to next-generation sequencing (NGS), Sanger sequencing, qPCR, ddPCR and microarrays, etc. BunnyMag DNA Purification & Size-Selection Beads uses high performance magnetic beads and a unique buffer system that allows for the adjustment of the ratio of magnetic beads to sample to achieve selection and purification of DNA fragments from 100bp-10kb.
BunnyMag DNA Purification & Size-Selection Beads is based on the SPRI (Solid Phase Reversible Immobilization) paramagnetic technology, which can selectively bind nucleic acid fragments of different sizes by adjusting the volume ratio between the beads and the sample to meet the needs of different length of nucleic acids, and are suitable for purification and screening of ideal size DNA fragments in high-throughput sequencing. The product is compatible with various brands of DNA and RNA library preparation kits, and can perfectly replace AMPure XP Beads and Axygen Beads, which are widely used in various NGS platforms.
High Recovery: Fragment recovery and library size highly consistent with competitive products.
High Repeatability: Specially developed for fragment screening, accurate and controllable fragment size, good reproducibility.
Broad Compatibility: Compatible with manual and automated process.
High Stability: Effective removal of excess dNTPs, residual primers, salt ion contamination.
Figure 1. The recovery efficiency of magnetic beads.
Use different ratios of BunnyMag DNA Purification & Size-Selection Beads and Ampure XP beads to purify and recover the same amount of DNA fragments. The recovery of BunnyMag DNA Purification & Size-Selection Beads is highly consistent with Ampure XP beads.
Figure 2. Accurate selection size of fragment.
For the initial size of 200-1500bp DNA fragments, the product is used to achieve accurate fragment selection by two-step magnetic bead selection and purification.
Figure 3. The fragment selection quality.
Using BunnyMag, Ampure XP and Axygen beads respectively, BunnyMag DNA Purification & Size-Selection Beads showed a high degree of consistency with competitive products in terms of fragment selection results under different selection ratio conditions.
Main Composition: Superparamagnetic beads, Polyethylene glycol (PEG) , NaCl.
Structure of magnetic beads (3-layer)
Innermost core is Polystyrene (PS).
Second layer wrapped with magnetic material——Fe3O4.
The outermost surface is a polymer modified by carboxyl groups (-COOH).
Store at 2-8°C upon arrival (not freeze), for up to 12 months.