BunnyMag Body Fluids Microbiome DNA Isolation Kit uses magnetic beads with a unique separation function and a unique buffer system. It is applicable for microbial DNA extraction from a variety of biological fluid samples (such as alveolar lavage, sputum, cerebrospinal fluid, etc.) & swab samples, and is compatible with host removal samples.
BunnyMag Body Fluids Microbiome DNA Isolation Kit is applicable for microbial DNA extraction from a variety of biological fluid samples (such as alveolar lavage, sputum, cerebrospinal fluid, etc.) and swab samples, and is compatible with host removal samples.
With advanced nucleic acid extraction technology, super para-magnetic beads and unique buffer system, the operation is safe and convenient, with maximum removal of protein, lipid and other impurities. The isolated DNA is suitable for a variety of downstream applications, including PCR, qPCR, mNGS library preparation, etc., providing efficient protection for research and clinical in vitro diagnosis.
High Quality: High DNA extraction yield, good purity, effective removal of impurities and downstream PCR inhibition factors, effective control of exogenous microbial contamination.
Unbiased: Unbiased microbial nucleic acid extraction, compatible with multiple pathogen types, minimizes microbial loss in samples.
Various Sample Types: Widely support alveolar lavage fluid, sputum, swabs and other biological fluid samples.
Broad Compatibility: Compatible with manual and automatic extraction programs, adapted to Tecan, Hamilton, PE and other nucleic acid extraction workstations.
Figure 1. Efficient recovery of pathogenic DNA from different liquid samples
Using sputum, alveolar lavage, and oral swab as test samples, BunnyMag Body Fluids Microbiome DNA Isolation Kit was used for microbial DNA extraction, comparing to similar products from Qiagen and T company. Our kit had high extraction yields in line with similar products from competing companies.
Figure 2. High library preparation yield
Using the BunnyMag Body Fluids Microbiome DNA Isolation Kit and T company's similar product, we extracted total microbial DNA from 5 clinical alveolar lavage samples and constructed libraries with equal quality, and the library preparation yield was higher than that of competing products.
Figure 3. Unbiased DNA extraction for different types of microbiome
Z microbial standards were used as test samples into 5 body fluid samples, and microbial DNA was extracted using BunnyMag Body Fluids Microbiome DNA Isolation Kit and Qiagen's similar products respectively. Also, library preparation and sequencing were completed. The figure displays that the product was able to extract different kinds of micro-organisms unbiasedly, and the extraction efficiency and accuracy were highly consistent with the competing products.
Lysis Binding Buffer
Guanidine isothiocyanate, 5 M
Washing Buffer 1
Guanidine isothiocyanate, 3 M
Washing Buffer 2
Ethanol absolute, 80%
20 mg/mL Proteinase K
Please take out the magnetic beads and proteinase K and store at 2-8℃, and others store at 10-30℃.
The validity period is 12 months.